Index
visionCATS
4.1
  • What’s new in visionCATS 4.1
    • Changelog
      • visionCATS 4.1
        • Enhancements
        • Bug fixes
      • visionCATS 4.0
        • Enhancements
        • Breaking changes
        • Bug fixes
      • visionCATS 3.2 SP 2
        • Enhancements
        • Bug fix
      • visionCATS 3.2 SP 1
      • visionCATS 3.2
        • Enhancements
        • Breaking changes
        • Bug fixes
      • visionCATS 3.1
        • Enhancements
        • Bug fixes
      • visionCATS 3.0
        • Enhancements
        • Bug fixes
      • visionCATS 2.5
        • Enhancements
        • Bug fixes
    • Technology update
      • .NET
      • New Windows services
      • New comparison image format
    • CAMAG® Automatic Developing Chamber 3 (ADC 3)
      • CAMAG® HPTLC PRO Module APPLICATION
      • CAMAG® TLC Scanner 4
    • Profiles view in the impurity test
    • Evaluation
    • Spectrum Library Only with Spectrum Library option
    • Known Issues and workarounds
  • User documentation
    • Main window
      • Main Toolbar
        • Title bar
        • Main toolbar and menu
      • Software and instruments help
        • Software
        • Instrument manuals
        • About visionCATS
      • Toolboxes
        • Instruments Toolbox
        • System Log Toolbox
      • System Status Bar
        • Last system log entry
        • HPTLC PRO SYSTEM status
        • Selection of the HPTLC PRO SYSTEM
        • Selected HPTLC PRO SYSTEM status
        • Login of current user
        • Name of the visionCATS server and status of all instrument services:
        • Used memory by the client
    • File Explorer
      • Main explorer view
        • 1.Contents of the current folder
        • 1. Navigation
        • 3. History
        • 4. Status bar
        • 5. Sort options
        • 6. Search options
          • Default textual search
          • Simple search shortcuts
          • Advanced search
            • Building search queries
            • Configuring the default search
            • Defining user queries
      • Preview view
      • File Types
        • Folders
        • Methods
        • Analysis
        • winCATS Analysis
        • Comparison
      • File Status
      • File Actions
        • Menu New
        • Menu File
        • Menu Edit
        • Menu Tags
      • Exchanging files with other visionCATS installations
        • Importing
          • Unsupported data
        • Exporting
    • Vials management
      • Vial ID naming
      • Reuse of vial
      • Additional vials management features
    • Method / Analysis File
      • HPTLC Steps
      • Chromatography
        • Track Assignment
          • Overview
          • Description of fields
          • Display
          • Keyboard navigation
          • Import/export functions
            • Options and formats
            • Fields and checks
            • Tracks import preview
        • Instrument step parameters
          • Plate Layout
            • Plate Manufacturer
            • Stationary Phase
            • Batch Number
            • Plate Format
            • Application Type
              • Application Position Y [mm]
              • First Application Position X [mm]
              • Track Distance [mm]
              • Application Length [mm]
              • Application Width
            • Solvent Front Position [mm]
          • CAMAG® HPTLC PRO Module APPLICATION
          • ATS 4
            • Sample Solvent Type
              • Equivalent Solvents
              • Dosage Speed [nL/s]
              • Pre-Dosage Volume [nL]
              • Filling Speed [µL/s]
              • Filling Vacuum Time [s]
              • Retraction Volume [nL]
              • Rinsing Vacuum Time [s]
            • Filling/Rinsing Quality
              • Rinsing Solvent
              • Rinsing Cycles
              • Do Not Rinse Before Next Vial
              • Empty Syringe Before Next Vial
              • Flush nozzle
              • Filling cycles
              • Fill Only Programmed Volume
              • Return Unused Sample into Vial
              • Syringe Volume [µL]
              • Spray Gas:
            • Rack Type
              • Vial Bottom Level [mm]
            • Nozzle Heating
              • Temperature Settings
          • Linomat 5
            • Sample Solvent Type
              • Equivalent Solvents
              • Dosage Speed [nL/s]
              • Pre-Dosage Volume [nL]
          • Manual application
          • CAMAG® HPTLC PRO Module DEVELOPMENT
          • ADC 2
          • ADC 3
          • AMD 2
          • Chamber
          • CAMAG® HPTLC PRO Module DERIVATIZATION
          • Derivatizer
          • Immersion
          • Spraying
          • TLC Visualizer
          • TLC Scanner 4
            • Scanner Type
            • Optimization For
            • Measurement Mode
            • Detector Mode
            • Speed/Resolution/Slit
              • Scanning Speed
              • Data Resolution
              • Slit
              • Partial Scan
            • Wavelength Selection
              • Lamp
              • Wavelength(s) Applied
            • Spectrum Parameters
              • Reference Spectrum
              • Purity
              • Lamp
            • Profile Representation
          • Common features
        • Instrument step execution
          • Instruction display
            • HPTLC PRO
          • Execution display
            • Common progression display
            • Closing analysis during execution
            • Application steps
            • Development steps
              • ADC 3
            • Data acquisition steps
              • TLC Visualizer
              • TLC Scanner 4
              • HPTLC PRO
          • Analyses recovery
        • Multiple application steps
        • Multiple development steps
        • Extended Linearity
      • Data View
        • Images View
          • DataView Toolbar
            • General tools
            • Export tool
            • 𝑅ꜰ Tools and Tracks
            • Image Settings
              • HDRI Settings
            • Annotations
        • Tracks View
        • Profiles View
      • Spectrum
        • Positioning
          • Overview
          • Spectrum Definition Table
          • Substance Positioning
        • Execution
        • Data
          • Overview
          • Spectrum Scan View
            • Display
            • Toolbar
            • Purity
            • Correlation
            • Maximums
            • Spectrum Library Only with Spectrum Library option
        • Correlation
          • Overview
          • Spectrum Correlation Data
      • System Suitability Testing (SST)
        • SST Table
          • Toolbar
        • Overview
        • SST View
      • Impurity test
        • Checks
          • Toolbar
          • Check computation
        • Tracks view
          • Overview
          • Data View
        • Profiles view
          • Overview
          • Data View
      • Evaluation
        • Evaluation steps
          • Definition
            • Overview
            • Substance Table
              • Advanced options
            • References
            • Samples
          • Integration
            • Overview
            • Peaks table
            • Profiles view
            • Integration tools
              • Bounds
              • Smoothing
              • Baseline
              • Profile Subtraction
              • Peaks
          • Substance assignment
            • Substances table
            • Profiles view
            • Substances view
            • Tracks view
            • Toolbar
          • Calibration
            • Overview
            • Height/Area views
            • Calibration function view
          • Results
            • Overview
            • Main results
              • Main display
              • Configuring the display
              • Limit tests
              • Sample reference amount display
            • Substances groups
              • Overview
              • Definition
              • Display
        • Advanced options
          • Related Substances
            • Overview
            • Defining main and related substances
            • During the calibration
            • In the results
          • Internal Standard
            • Overview
            • Defining the Internal Standard
            • During the calibration
            • In the results
          • Standard for error correction
            • Overview
            • Defining the standard for error correction
            • During the calibration
            • In the results
          • Reproducibility
            • Overview
            • During the definition
            • In the results
          • Assignment mode
            • Overview
            • Multiple assignment mode
              • Integration phase
              • Substance assignment phase
              • Calibration phase
              • Results phase
            • Range assignment mode
              • Integration phase
              • Substance assignment phase
              • Calibration and results phases
          • LOD / LOQ
            • Overview
            • Defining LOD/LOQ
            • Calibration and results
              • USP mode
              • LOD checks
              • LOQ checks
        • Calibration computations
          • Concepts and common values
            • Regression functions
            • Regression range
            • Computation of the analysis points
            • Coefficient of variation of the calibration function
            • Correlation coefficient of the calibration function
            • Coefficient of variation of the sample results
          • Computation of the calibration functions
            • Linear-1
              • Calibration function
              • Regression
            • Linear-2
              • Calibration function
              • Regression
            • Polynomial
              • Calibration function
              • Regression
            • Mime-1 and Mime-2
              • Principles
              • Starting point
              • Calibration function
              • Regression
        • Evaluation assistant
      • Remarks
        • Editing features
        • Limitations
      • Report
        • Report Settings
          • Report template selection
          • Report template options
          • Tracks data options
          • Data acquisition options
          • Image display options
          • Evaluation options
          • Log options
        • Outline
        • Preview
        • Method report
      • Log
      • Export of data
        • Peaks table export
        • Substance assignment export
        • Evaluation results export
        • Profile data export Only with Export data option
        • Raw profiles export Only with Export data option
        • Integrated profiles export Only with Export data option
        • Comparison profiles export Only with Export data option
        • Spectrum export Only with Export data option
        • Analysis spectrum data export Only with Export data option
        • Comparison spectrum export Only with Export data option
    • Comparison Viewer
      • Overview
        • How it works
        • Common functionalities
      • Export to comparison
        • Overview
        • Images and profiles export to comparison
        • Spectrum export to comparison
      • Image comparison
        • Display
        • Overview
        • Illumination selection
        • Toolbar
        • Track information
        • Image format
      • Profile comparison
        • Tracks view
          • Display
            • Overlay
            • Stack
          • Overview
          • Wavelength or illumination selection
          • Toolbar
          • Tools
          • Profile selection
            • Profile selection toolbar
            • Profile selection lists
        • Wavelengths view
      • Spectrum comparison
        • Display
        • Overview
        • Spectra to display
        • Toolbar
        • Maximums
        • Spectrum definition selection
      • Remarks
      • Report
        • Report template selection
        • Report settings
          • Global settings
          • Image settings
          • Profile settings
          • Spectrum settings
      • Log
    • Tools
      • Toolboxes
      • Other tools
        • Vial/Substance Editor
          • Vial List
          • Substance List
          • Selected Vial
          • Selected Substance
          • Substance in Vial
          • Bulk editing
        • Global lists editor
          • Global list items management
          • Toolbar
          • Used in section
          • Naming checks
            • Adding and renaming items
            • Global list items formatting
            • Global list items display configuration
          • Migration from visionCATS 3.2
            • Updated built-in Items (old name -> new name)
            • New global list Known chemicals
            • Files import
            • Handling of duplicates
    • Administration / Options
      • User Management
        • Users and groups rights
        • Users
          • Toolbar
          • User table
            • Tooltips
        • Groups
          • Toolbar
            • New group
      • General Settings
        • Installations
          • Toolbar
          • Main installation table
        • COM Ports
          • Toolbar
        • USB Ports
          • Toolbar
        • Instruments Configuration
        • Global Configuration
          • Analysis
            • Exposure normalization for images
            • Exposure normalization range on track
            • TLC Visualizer 2/3 use the same reference point as a TLC Scanner 3/4
            • TLC Visualizer (any version) use a smaller ROI for auto exposure
            • TLC Visualizer (any version) max. exposure time for HDRI images
          • User management
          • Notifications
          • Global Lists
      • User Options
        • Miscellaneous
          • Explorer options
          • Method and analysis options
          • Dialogs options
          • CSV export options
        • Notifications
      • Report Settings
        • Reports Configuration
          • Reports header logo
          • Reports page setup
          • Global CSS
        • Report templates
        • Report templating
          • Technologies
            • HTML
            • CSS
              • Colors
              • SCSS
            • JavaScript
              • Handlebars
              • linq.js
              • D3.js
              • Flot (legacy)
              • Other JavaScript libraries
            • EO.Pdf
          • Breaking changes
            • visionCATS 4.1
              • SCSS files
            • visionCATS 4.0
              • JSON
              • Report data
              • SCSS files
            • visionCATS 3.2
              • Evaluation results
            • visionCATS 3.1
              • CSS files
              • Libraries changes
              • Rendering changes
              • D3.js replacing Flot
              • JavaScript version
              • HTML changes
            • visionCATS 3.0
              • New instrument names
            • visionCATS 2.5
              • Unit converter functions
              • Sample reference amount
          • Versioning
          • Debugging the report template
            • PDF generation debug
            • PDF generation temporary folder
              • Report temporary files structure
            • Debugging tools
              • Web browser developer tools
              • Code editor
          • Changing template styles
            • Global and template specific styles
            • Tutorials
              • Changing the global styles
              • Overriding global styles for a specific template
          • Changing template structure
            • General structure
            • Heading and report overview
            • Tutorials
              • Simple re-ordering and visibility
              • Header logo only on the first page
              • New layout
              • Displaying only log of a specific type
              • Bullet graph for limit tests
    • Profiles viewer
      • Axis
      • Navigation in 2D
        • Zooming the view
          • One axis zooming
        • Panning the view
      • Navigation in 3D
        • Zooming the view
        • Panning the view
        • Rotating the view
      • Viewing modes
      • Export
    • HPTLC PRO
      • Overview
        • Terminology
        • Status colors
          • CAMAG® HPTLC PRO SYSTEM status
          • HPTLC PRO Module status
        • Conveyor Manager
      • Configuration
        • Setup
        • HPTLC PRO Settings
          • CAMAG® HPTLC PRO SYSTEM Configuration
            • Main toolbar
            • HPTLC PRO SYSTEM row
            • HPTLC PRO SYSTEM toolbar
        • HPTLC PRO Module
          • Properties tab
          • Manual control tab
            • CAMAG® HPTLC PRO Module PLATE STORAGE
            • CAMAG® HPTLC PRO Module APPLICATION
            • CAMAG® HPTLC PRO Module DEVELOPMENT
            • CAMAG® HPTLC PRO Module DERIVATIZATION
          • Diagnostics tab
          • Operations tab
          • Firmware tab
        • CAMAG® HPTLC PRO SYSTEM Details
          • Display
          • History tab
          • Manual control tab
          • Diagnostics tab
            • With CAMAG® HPTLC PRO Module PLATE STORAGE
      • Execution
        • Typical execution
        • Preparation
          • Analysis splitting in HPTLC/TLC steps and HPTLC PRO runs
          • Multiple CAMAG® HPTLC PRO Module APPLICATION steps in an analysis
        • Execution
          • Display Tab overview
            • Toolbar
            • Modules
            • Status
            • Sequences
          • Consumables validation
            • Consumables check
              • Vial position in HPTLC PRO Module APPLICATION rack
          • Execution of a sequence
            • Using an CAMAG® HPTLC PRO SYSTEM without any CAMAG® HPTLC PRO Module PLATE STORAGE
            • Using an HPTLC PRO SYSTEM with a CAMAG® HPTLC PRO Module PLATE STORAGE
          • Last sequence info
          • History tab
            • Functionalities
            • Table display mode
            • Types of elements
            • Groups
            • Event categories
            • Action categories
            • Severity
        • Troubleshooting
          • Cases
          • Troubleshooting functions
            • HPTLC PRO SYSTEM reinit
            • HPTLC PRO Module reset
            • HPTLC PRO SYSTEM reset
      • Advanced
        • Understanding the HPTLC PRO Module and CAMAG® HPTLC PRO SYSTEM initialization
    • 21CFR Part 11 compliance
      • Understanding the logging features
      • System logger
      • HPTLC Logger
      • Deletion of data
        • Files and folders archiving
        • Steps data loss and steps reexecution
      • Motivated change
      • Open as management
      • E-Signature
      • Data integrity
        • How to setup Windows user rights for data integrity
  • Installation and support
    • Introduction
      • Architecture
      • Editions and options
      • End user license information
    • Installation
      • Parts supplied
        • Content of the visionCATS installation media
      • PC System Requirements
        • System Requirements
        • Installation Drive
        • Communication ports
        • Supported operating systems
          • Needed software
        • Network
        • Color quality
        • System printer (optional)
        • Additional software installed
      • Installation procedure
        • Post installation
      • Troubleshooting
      • Post Installation
        • Graphics performance preference
        • Disable Fast startup
        • Disable Windows hibernation and sleep modes
        • Disable USB selective suspend setting
        • Inform users to close visionCATS before locking the session
      • Installation of USB to RS232 adapter
        • Troubleshooting for RS232 adapter
      • Installation of TLC Visualizer 2/3 camera
        • Camera driver installation
      • Installation of HPTLC PRO drivers
        • USB Driver
          • Automatic installation by Windows Update
          • Manual installation
          • Installation end
        • DFU Driver
      • Update of TLC/HPTLC instrument firmware
        • For instruments with RS232 connection
      • Update of HPTLC PRO Module firmware
      • Update of HPTLC USB instrument firmware
    • Licensing
      • General information
      • Activation
      • Upgrades
        • Re-activating your existing license
      • Hardware changes
    • Client profiles
    • Instrument diagnostics
      • Diagnostics expiration for HPTLC PRO Module
      • Diagnostics view
      • How to do the diagnostics
    • visionCATS Diagnostics
      • Properties
      • Client Diagnostics
    • Backup / Restore
      • Backup procedure for visionCATS
      • Restore procedure for visionCATS
      • Scheduling backup
        • Automatic backup
        • Manual backup
          • Configure network share for scheduled backup
            • Network architecture
            • Setting backup folder
            • Creating the network share with correct rights
            • Setting backup folder: retry
    • Instrument Properties
    • Maintenance and troubleshooting
      • General maintenance
      • Service Tool
    • Known Issues and Workarounds
      • Troubleshooting USB connection errors for HPTLC PRO Modules or new HPTLC instruments
    • Logging
      • Installer logs
      • visionCATS logs available
      • The Get debug logs functionality
  • visionCATS Guide
    • General Introduction
      • The visionCATS concept: HPTLC made easy!
      • Key features
      • Benefits
    • Getting Started
      • Main window
      • Folders
    • Create a method
      • Working with methods that comply with USP and Ph. Eur.
      • Working with methods from the Method Library
      • Create a new method from scratch
    • Execute an analysis
      • Performing a method transfer validation
      • Setting the analysis
    • Data view
      • Data type
      • Overview
      • Toolbar
      • Remarks, report, log
      • Relevant tools
      • Export of Images
    • Working with SST
    • Scanning Densitometry
      • Single-wavelength scan
      • Multi-wavelength scan
    • Peak profile from images (PPI)
    • Evaluation
      • Basics of quantitative evaluation in visionCATS
      • Manual mode
      • Expert mode
      • Peak table export
      • Related substances
      • Standard for error correction
      • Reproducibility
      • Limit test
      • Export results
      • Export graphs
    • Spectrum Scan
    • Working with Comparison files
      • Image Comparison
      • Profile Comparison
      • Spectrum Comparison
    • Other features
      • Quick scan / Partial scan
      • Dual Wavelength scan and (blank) track subtraction
      • AMD 2 settings
    • Reports
    • Export of Data
    • 21 CFR Part 11
    • Example Files
    • Case studies with application tutorial videos
      • HPTLC-Fingerprint of Ginkgo biloba flavonoids (qualitative example)
      • Identification of fixed oils by HPTLC (qualitative example, including method transfer validation)
      • In-process control during chemical synthesis (qualitative example, including HPTLC-MS)
      • Quantitative determination of steviol glycosides (quantitative example)
  • Glossary
visionCATS
  • User documentation
  • Method / Analysis File
  • Evaluation
  • Advanced options
  • Related Substances

Related Substances

Overview

The Related Substances functionality allows you to calibrate a substance of an evaluation with the calibration curve of another substance. A typical use case consists in the quantification of impurities in drug substances. The Main substance is the main component of the drug while each impurity is a Related substance. In order to confirm that the related substances are impurities, the sum of their quantities must not exceed a defined limit (normally 2% of the main substance). Therefore the typical workflow consists in:

  • Quantifying the main substance at an appropriate set of concentration/volume applied,

  • Quantifying the related substances at an appropriate set of concentration/volume applied, normally smaller than the set used for the main substance, and

  • Using the calibration curve of the main substance for the calibration of the related substances.

Defining main and related substances

../../../_images/related_substances_definition.png

In Definition tab of the evaluation, use the Advanced options button to make the Related substances field appear. In an evaluation, there can be only one Main substance, other substances can be of type Related substances or None (no related substance). Of course, the functionality will only work if the main substance and at least one related substance are defined. Because the related substances use the calibration curve of the main substance, the calibration-related fields are hidden for related substances.

The related substance functionality can be used in 3 modes, accessible by clicking on the Related substances parameters button :

  • Default: this is the simplest and the most generic mode, already present in winCATS, where only the substitution of the calibration curve is done for each related substance. This mode offers the most freedom but gives only the normal results of the quantification. The 2 other modes include the Allowed impurity amount field which enables the computation of the sum of impurities for each track.

  • By dilution: use this mode when a vial defined as Sample in the evaluation has been diluted (one or many times). Each dilution gives its own vial ID defined as Reference in the evaluation. In the configuration of this mode:

../../../_images/related_substances_dilution.png

The Sample to use as reference concentration field has to be filled and the corresponding Amount and Volume solution of the sample in the Samples section have to be defined.

../../../_images/related_substances_dilution_sample.png

The Amount and Volume solution fields give a reference concentration, and the volume applied has to be the same for all applications of this sample, therefore, there’s a reference quantity too, which will be used when computing the results of the evaluation. In the References section, once the reference concentration is defined, the dilution factor can be directly entered instead of the concentration, and the tooltip explains the correspondence between the dilution factor and the actual concentration:

../../../_images/related_substances_dilution_reference.png

  • By volume: use this mode when the same vial is used on many reference tracks in the evaluation at different volumes. A correspondence between one of those reference application and the known amount of impurities (in %) has to be defined:

../../../_images/related_substances_volume.png

Similar to the By dilution mode, this configuration actually gives a reference quantity which will be used as a basis to compute the results in percentage.

During the calibration

During the Calibration phase:

  • The calibration configuration on top of the view is not modifiable and shows the calibration data of the main substance

  • An information text recalls the main substance

  • The tooltip on a reference or sample calibration point shows the additional relative quantity computed (only in By dilution and By volume modes)

  • The x-axis is expressed in percentage (only in By dilution and By volume modes)

../../../_images/related_substances_calibration.png

In the results

In the Results tab, the results are not displayed for the main substance of the evaluation. When the By dilution or the By volume mode is selected, a Related substances tab appears. It displays, for each track, the details of the related substances percentages. In order to confirm that the related substances are impurities, the Status field indicates whether some tracks exceed the allowed impurity amount.

The result of the computation of reference amount is displayed and the details is explained into a tooltip:

  • For By dilution

../../../_images/related_substances_results_dilution.png
  • For By volume

../../../_images/related_substances_results_volume.png

Use the Export results (CSV) button to get a flattened table of the related substances results useful for future processing.

../../../_images/related_substances_results.png
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© Copyright 2025, CAMAG. Last updated on Feb 21, 2025.

visionCATS version: 4.1
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4.1